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you can find tyrosine (Y223) and lysine (K139) residues that may also be contributing towards the interaction While using the phosphate moiety with the substrate. Deprotonation of either of those residues at higher pH could lead to a drop in the value of k

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cat vs. pH-profile revealed in Determine 2A for Zn-NagA was suit to equation three and these benefits suggest the deprotonation of an individual acid using a kinetic p

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should be appreciably various Because the H×H motif from your end of β-stand 1 is replaced by Q×N and therefore it is highly very likely this enzyme can bind only a single divalent cation from the Lively web-site.

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